UnipicK+ is a universal platform for tissue microdissection, single cell acquisition, and deposition into the single wells for further molecular analysis or clonal expansion. The main working principle is based on the capillary based vacuum pulse assisted technology (CTAS; Kudo et al, 2012). Developed system can microdissect tissue sections, collect and deposit individual cells from any adherent cultures grown in standard cell culture dishes in as small as 15 nl volume, compatible with various downstream single cell analyses and next generation sequencing. Like UnipicK, the system may be used with a wide range of inverted microscopes. The benefits include cost-efficiency, simple operation, digital interface, complete workflow from single cell isolation to downstream analysis, compatibility with a wide range of inverted microscopes and use of standard plates and culture dishes.